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KMID : 0616120080390040290
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Korean Journal of Pharmacognosy
2008 Volume.39 No. 4 p.290 ~ p.294
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Anti-oxidative Effect of Some Plant Extracts Against Nitric Oxide-induced Oxidative Stress on Neuronal Cell
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Koo Uk
Lee Hak-Ju
Lee Dong-Ho
Lee Hyun-Jung
Ham Ah-Rom
Cho Eun-Young
Mar Woong-Chon
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Abstract
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The objective of this study is screening the anti-oxidative effects of several plant MeOH extracts against oxidative stress in Neuroblastoma cell. Oxidative stress has been implicated in the pathogenesis of many neurotoxicity, neurodegenerative disorders and cell death. This oxidative stress is generated by ROS (Reactive Oxygen Species) such as nitric oxide, nitrogen dioxide, peroxyl, superoxide (O2 - ), hydroxyl, alkoxyl. So, in the present study, we induced oxidative stress by treatment of sodium nitroprusside (2.5 mM) in human neuroblastoma SH-SY5Y cell which was treated samples before 24hr, and cell viability was measured by MTT reduction assay. Of those tested, the extracts of Paeonia japonica (roots), Eucommia ulmoides (炒)(barks), Paeonia japonica (曝乾)(roots), Phyllostachys bambusoides (stems), Polygala tenuifolia (去心, 炒)(roots), Paeonia japonica (roots), Polygala tenuifolia (roots), Machilus thunbergii (barks), Mallotus japonicus (leaves), Poria cocos (whole), Sophora flavescens (roots), Angelica tenuissima (roots), Angelica gigas (當歸尾)(roots) showed anti-oxidative effects[EC50<15.20 μg/ml(Carnosine:Positive control)]in dose dependent manner.
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KEYWORD
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Sodium nitroprusside, Oxidative stress, Anti-oxidative effects, MTT reduction assay, EC50
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